Marco Bezzecchi delivered a qualifying performance that silenced the Ducati-dominated paddock. The championship leader smashed the all-time Mugello lap record in Q2 to head an all-Aprilia front row, with Raul Fernandez second after coming through Q1 and factory teammate Martin third. Reigning champion Marc Marquez returned to fourth on the grid, joined on an all-Ducati second row by Aldeguer and Bagnaia. Aprilia had reversed the Friday order completely. The Mugello crowd noticed.

